博士后(中美联合梅毒研究项目)

1、Job Description
Position:Postdoctoral Researche
Specialization:Pathogenic Microbiology, Molecular Biology, Structural Biology, etc.
Minimum Educational Qualification: Ph.D.
Work Experienc:No specific requirement, candidates with experience studying in the United States are preferred

2、International Exchange
During the station period, you can conduct research projects at the Spirochete Research Laboratories of the University of Connecticut, Connecticut Children’s - Division of Infectious Diseases, and UConn Health - Departments of Pediatrics and Medicine, under the guidance of Prof. Justin Radolf.
在站期间可前往美国康涅狄格大学Spirochete Research Laboratories Connecticut Children’s - Division of Infectious Diseases & UConn Health - Departments of Pediatrics and Medicine实验室在Prof. Justin Radolf指导下进行课题研究。


3、Job Responsibilities
Participate in the collaborative project between the National Institutes of Health (NIH) and China, titled "Global sequence and surface antigenic diversity of Treponema pallidum outer membrane proteins" (UCHC7-122226512). Specifically, conduct research on the development of nanovaccines by combining single-cell omics sequencing technologies and bio-tracing techniques to screen for syphilis disease progression biomarkers and protective antibodies.
Familiarity with clinical aspects of syphilis and its immunopathogenic mechanisms,as well as foundational experiments and animal experiments related to syphilis.
Prokaryotic expression and purification of key ECL (extracellular loop) of Treponema pallidum candidate proteins.
Detection of the reaction between key ECL and antibodies from different patient sera.
Use bioinformatics tools to analyze the genomic polymorphism of Treponema pallidum, focusing on the variation of outer membrane proteins.
Preparation of monoclonal antibodies from BCR (B cell receptor) sequences of skin lesions.
Screen and validate antibodies obtained from the existing syphilis protein library. Use the obtained antibodies to screen, identify, and analyze unknown syphilis proteins.
Other research related to syphilis.

4、Job Requirements
Ph.D. graduate in relevant majors such as pathogenic microbiology, molecular biology, structural biology, etc.
Experience in recombinant protein expression using expression systems like Escherichia coli and mammalian cells, as well as related work in expression system selection, purification, antigen/antibody production, and identification. Experience in the study of bacterial outer membrane proteins is preferred.
Basic understanding of bioinformatics.
Strong research literacy, ability to plan and execute experiments, solve experimental problems, and analyze results.
Strong teamwork, communication, coordination, and expression skills.